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pierce bca protein assay kit  (Beyotime)
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Beyotime pierce bca protein assay kit
Pierce Bca Protein Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pierce bca protein assay kit  (tiangen biotech co)
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Pierce Bca Protein Assay Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pierce bca protein assay kit  (Avantor)
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Avantor pierce bca protein assay kit
Pierce Bca Protein Assay Kit, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pierce bca protein assay kit  (Beijing Solarbio Science)
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Beijing Solarbio Science pierce bca protein assay kit
nsPEFs enhanced the release <t>of</t> <t>EVs</t> from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using <t>BCA</t> method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.
Pierce Bca Protein Assay Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bca kit  (Becton Dickinson)
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nsPEFs enhanced the release <t>of</t> <t>EVs</t> from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using <t>BCA</t> method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.
Bca Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pierce® bca protein assay kit  (Jiancheng Inc)
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nsPEFs enhanced the release <t>of</t> <t>EVs</t> from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using <t>BCA</t> method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.
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pierce® bca protein assay kit  (KeyGene Inc)
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nsPEFs enhanced the release <t>of</t> <t>EVs</t> from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using <t>BCA</t> method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.
Pierce® Bca Protein Assay Kit, supplied by KeyGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pierce bca protein assay kit  (Beijing CWBio)
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nsPEFs enhanced the release <t>of</t> <t>EVs</t> from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using <t>BCA</t> method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.
Pierce Bca Protein Assay Kit, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pierce™ rapid gold bca protein assay kit  (Fisher Scientific)
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Fisher Scientific pierce™ rapid gold bca protein assay kit
nsPEFs enhanced the release <t>of</t> <t>EVs</t> from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using <t>BCA</t> method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.
Pierce™ Rapid Gold Bca Protein Assay Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pierce bca protein assay kit  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
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nsPEFs enhanced the release <t>of</t> <t>EVs</t> from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using <t>BCA</t> method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.
Pierce Bca Protein Assay Kit, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pierce† bca protein assay kit  (Keygen Biotech)
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nsPEFs enhanced the release <t>of</t> <t>EVs</t> from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using <t>BCA</t> method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.
Pierce† Bca Protein Assay Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pierce bca protein assay kit  (GenStar Biosolutions)
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nsPEFs enhanced the release <t>of</t> <t>EVs</t> from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using <t>BCA</t> method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.
Pierce Bca Protein Assay Kit, supplied by GenStar Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nsPEFs enhanced the release of EVs from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using BCA method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.

Journal: Journal of Orthopaedic Translation

Article Title: Functionally improved mesenchymal stem cells via nanosecond pulsed electric fields for better treatment of osteoarthritis

doi: 10.1016/j.jot.2024.03.006

Figure Lengend Snippet: nsPEFs enhanced the release of EVs from MSCs and EVs-nsPEFs enhanced viability, proliferation and migration of chondrocytes and reversed the change of cartilage markers in OA-like chondrocytes. (A) Morphology of EVs and EVs-nsPEFs under TEM. Scale bars, 200 nm. (B) The number of EVs in random field (n = 6) was counted. (C) EVs size distributions and particle concentration analyzed by NTA. (D) Analysis changes of particle concentration between EVs and EVs-nsPEFs. (E) Analysis changes of the protein concentration between EVs and EVs-nsPEFs using BCA method. (F) WB results for the EVs markers CD9, Alix, TSG101 and the negative marker Calnexin. (G) Representative bioluminescence images for Red dye Dil-labeled EVs and EVs-nsPEFs in chondrocytes at 6 h, 12 h and 24 h. Scale bars, 50 μm. (H) CCK8 assay to determine the viability of chondrocytes co-cultured with EVs and EVs-nsPEFs (n = 6). (I) Representative images of EDU staining of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 200 μm. (J) Representative images of transwell migration assay of chondrocytes co-cultured with EVs and EVs-nsPEFs. Scale bars, 100 μm. (K) Quantitative analysis of positive cells by EDU staining (n = 4). (L) Quantitative analysis of migrated cells by transwell assay (n = 4). (M) Schematic diagram of experimental design. (N) Representative images of immunofluorescence assay to detect the expression of Collagen II and MMP13 in chondrocytes in control (Group Normal), OA-like chondrocytes (Group IL-1β), OA-like chondrocytes co-cultured with EVs (Group EVs) or EVs-nsPEFs (Group EVs-nsPEFs). Scale bars, 50 μm. Quantitative analysis of immunofluorescence assay to detect the expression of (O) Collagen II and (P) MMP13 in chondrocytes (n = 4). * P < 0.05, ** P < 0.01, **** P < 0.0001. Bar labeled with different lowercase letters indicate a significant difference.

Article Snippet: According to the manufacturer's instructions, EVs Protein lysate concentrations were determined using a Pierce BCA Protein Assay Kit (Solarbio, China).

Techniques: Migration, Concentration Assay, Protein Concentration, Marker, Labeling, CCK-8 Assay, Cell Culture, Staining, Transwell Migration Assay, Transwell Assay, Immunofluorescence, Expressing, Control